Journal: Scientific reports

Article Title: let-7i-5p, miR-181a-2-3p and EGF/PI3K/SOX2 axis coordinate to maintain cancer stem cell population in cervical cancer.

doi: 10.1038/s41598-018-26292-w

Figure Lengend Snippet: Figure 4. miRNA profiling in CaSki CSCs and validation of differentially expressed miRNAs. (a) The small RNA sequencing was carried out for CaSki cells and CSCs enriched from CaSki cell line. This experiment identified 42 differentially expressed miRNAs (12 downregulated and 30 upregulated) in CSCs with a p value < 0.05. As per the miRNA nomenclature guidelines, the mature miRNA sequences are mentioned as miR- and precursor sequences as mir-. (b) miRNAs (let-7i-5p, miR-181a-2-3p, miR-615-3p, and miR-663a) were selected from the list of differentially expressed miRNAs for further validation. The expression of these miRNAs were analyzed by real time PCR and found to be in concordance with the data obtained from small RNA sequencing experiment. *p value < 0.05.

Article Snippet: The resulting plasmid was referred to as shSOX2. miRNA expression plasmids for the exogenous expression of miR-181a-2-3p (SC400203) and let-7i-5p (SC400011) were purchased from OriGene Technologies, Inc.

Techniques: Biomarker Discovery, RNA Sequencing, Expressing, Real-time Polymerase Chain Reaction